The risk corynespora cassiicola would pose to california is evaluated below. Occurrence of corynespora cassiicola isolates resistant to. Differentiation of corynespora cassiicola and cercospora sp. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms appeared on seedlings planted in late may and in june. A new foliar disease, corynespora leaf spot, has been detected in arkansas cotton travis faske extension plant pathologist corynespora leaf spot has been detected in several commercial cotton fields on a few varieties fm 1944, st 5458, and phy 499 in. Among them, 12, 34, 56, and 78 were pairs of epimers. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis. The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. Corynespora cassiicola from soybean to carbendazim and prothioconazole.
It is a reemerge pathogen on cotton in southeastern us. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is. Pdf symptomatology, hosts, and pathogenicity of corynespora. Pathogenicity of corynespora cassiicola on soybean canadian. Transformation of corynespora cassiicola by agrobacterium. Corynespora cassiicola from cotton and soybean in the southeastern united. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a. Hofs inhibit corynespora cassiicola growth in vitro and protect glycine max plants against this pathogen. Phylloplane fungi % biological control % corynespora % rubber % antagonism. Arvind kumar kurre, meghchand dewangan and dantre, r. No known resistance to target spot is currently available in commercial cultivars. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and has.
Metconazole exhibited excellent preventive and curative activities in vivo metconazole reduced disease severity of corynespora. The yellowish area surrounding the lesion can be seen only against the light. Initial characterization of corynespora cassiicola affecting florida tomatoes. Detection of corynespora cassiicola in hevea rubber tree. Frontiers genomewide analysis of corynespora cassiicola. The molecular detection of corynespora cassiicola on. Twelve new chromone derivatives, corynechromones al 112, were isolated from the spongederived fungus corynespora cassiicola xs200900i7. Pdf effects of corynespora cassiicola on lantana camara. Differentiation of corynespora cassiicola and cercospora. Purification and characterization of cassiicolin, the toxin produced by corynespora cassiicola, causal agent of the leaf fall disease of rubber tree. The planar structures were determined by extensive nmr spectroscopic data. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis cmv cucumber mosaic virus prsv papaya ringspot virus. Sobers the first report of corynespora cassiicola berk. Evaluation of fungicides against target leaf spot caused by corynespora cassiicola.
Whole genome sequences of four cotton and one soybean isolate from tennessee were used to develop single nucleotide polymorphism markers for cotton isolates. The isolation ofum591 from a patients contact lens indicates the pathogenic. Diversity of the cassiicolin gene in corynespora cassiicola and relation with the pathogenicity in hevea brasiliensis. In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. These climatic conditions would limit the ability of the pathogen to.
The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Corynespora cassiicola corycaoverview eppo global database. However, no polymorphisms were found at the its sequences. Pdf on sep 10, 2012, jose ricardo liberato and others published. Several lineages of this pathogen have been recognized, but the classification of isolates into clades is timeconsuming and still sometimes leads to. A new leaf and berry spot disease, and leaf fall caused by corynespora cassiicola is reported on coffea canephora in brazil. Few studies referred to the formation of chlamydospores by c. The effect of red light irradiation on development of corynespora leaf spot of cucumber plants cucumis sativus l. Genomewide analysis of corynespora cassiicola leaf. Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. Continuing to use this website means you agree to our use of cookies. Cotton isolates had little diversity at the genome level and very.
Evaluation of fungicides against target leaf spot caused. Gao wei 1,2, li baoju 2, wang wanli 1, hao yongjuan 1, shi yanxia 2. Chemical investigation of the ethyl acetate extract of corynespora cassiicola, isolated from leaf tissues of the chinese mangrove medicinal plant laguncularia racemosa, yielded four new secondary. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola. The absolute configurations of 110 were assigned by the modified moshers method and tddft ecd calculations. Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Two single nucleotide polymorphisms snps were detected in the internal transcribed spacer region of the nuclear ribosomal dna rdnaits of 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in. Genetic determinism of sensitivity to corynespora cassiicola. Growth inhibition was evaluated measuring the diameter of the. In the last few decades it has become one of the most destructive foliar and fruit diseases of tomato in the state mackenzie et al. Detection of corynespora cassiicola in hevea rubber tree from. Subcutaneous infection caused by corynespora cassiicola, a. Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was.
Clonality and geographic structure of hostspecialized. Corynespora cassiicola requires high humidity for infection and is additionally favored in locations with substantial periods of high moisture 1644 hours. Pdf sensitivity of corynespora cassiicola from soybean to. A new record of leaf spot disease on aquilaria malaccensis lamk in india. Structural analysis of cassiicolin, a hostselective. The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and gene. Pdf intraspecific variability of corynespora cassiicola. Here we confirm the occurrence of this species as an agent of subcutaneous phaeohyphomycosis. Frequency distribution of ec 50 values for tetramycin against 91 corynespora cassiicola isolates obtained from different cities in shandong, as based on a mycelial growth, b spore germination, and c germ tube elongation at several concentrations. Eco ri, msp i and rsa i endonucleases identified all isolates as corynespora cassiicola. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior histo ry of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on.
Red, lesions enlarged, coalesced, and finally covered the entire leaves of cucumber. Transcriptome and mirna analyses of the response to. Evaluation phylloplane fungi as biocontrol agent of corynespora lea. Suppression by red light irradiation of corynespora leaf. Documents about corynespora cassiicola coryca this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Growth and sporulation of the fungus on several media. On the lower surface, small, light brownish, somewhat soakedwater lesions appear first. Diagnostic features of corynespora cassiicola and its. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. Frontiers compounds released by the biocontrol yeast. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure.
Chemical investigation of the ethyl acetate extract of corynespora cassiicola, isolated from leaf tissues of the chinese mangrove medicinal plant laguncularia racemosa, yielded four new secondary metabolites, including three decalactones, xestodecalactones df as well as corynesidone c 4, in addition to four known compounds. Diagnostic features of corynespora cassiicola and its associated diseases identifying c. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is observed, or new strains evolve. The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc. Mar 28, 2017 corynespora cassiicola was consistently isolated from the leaf spots on inoculated lettuce plants following the same method that was used for the initial isolations. Only a single, historical clinical case of human infection caused by c. Although variation in aggressiveness to cotton and tomato were observed, no genetic diversity. A boll rot of cotton caused by corynespora cassiicola in.
Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to. Similarly, the phylogenetic analysis of 30 isolates of c. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole article pdf available in tropical plant pathology 385. B hyphae with septa black arrow in the edematous papillary dermis periodic. Corynespora cassiicola is a common pathogen of grasses and other herbaceous plants in the tropics 3,4.
Corynespora brown spot of papaya corynespora cassiicola. Strobilurins and related compounds are prone to the development of resistance and should be rotated with other chemistries. Borah r k, ahmed f s, sarmah g s, bhabesh gogoi, 2012. Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. Pdf first report of corynespora cassiicola causing leaf and. Decalactone derivatives from corynespora cassiicola, an. Genomic insight into pathogenicity of dematiaceous fungus.
Corynespora cassiicola corycadocuments eppo global database. Corynespora cassiicola is an important pathogen because its responsible for a considerable loss of crop production and yield. Corynespora cassiicola is a destructive plantpathogenic fungus causing widespread target spot epidemics, including outbreaks on cotton, soybean, and tomato in the southeastern united states. This fungus is present in some hevea rubber plantations and nurseries in. Corynespora cassiicola is the causal organism of the most devastating leaf disease of rubber in sri lanka. Diversity of the cassiicolin gene in corynespora cassiicola. Structural analysis of cassiicolin, a hostselective protein toxin from corynespora cassiicola. Target spot of tomato, caused by corynespora cassiicola, was first reported in florida in 1972. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior history of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on yba agar medium.
The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and. Aug 31, 2018 corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Later, the lesions become brown, necrotic, slightly sunken, with brownreddish margins. Jsv provided expertise for the manual tuning of clustering parameters. Growth sensitivity of corynespora cassiicola to thiophanate. Our raw reads are also published to sra at ncbi for bulk download needs.
Corynespora cassiicola appeared to be a minor pathogen for okra. Morphological and molecular characterization of isolates. Several lineages of this pathogen have been recognized, but the classification of isolates into clades is timeconsuming and still sometimes leads to unclear results. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Corynespora cassiicola attackes diverse agriculturally important plants, including soybean and cotton, in the us. Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to cercospora sp.
He found that the fungus was pathogenic to leaves of hydrangea, hydrangea macrophylla ser. We are setup to allow easy downloads of individual projects and dont provide means to download whole collections of data. Detection of corynespora cassiicola in soil with realtime quantitative pcr. Pdf corynespora brown spot of papaya corynespora cassiicola. Corynespora cassiicola is a species of fungus well known as a plant pathogen. Pdf infection process of corynespora cassiicola tagged.
Download fulltext pdf download fulltext pdf physiological characterization of corynespora cassiicola isolates article pdf available in tropical plant pathology 384. A new boll rot disease of cotton gossypium hirsutum l. Initial characterization of corynespora cassiicola affecting florida tomatoes pdf. Cotton, cowpea, cucumber, eggplant, sesame, soybean, tobacco. Corynespora cassiicola, which causes corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of hevea rubber trees in asian and african countries. For your convenience and fast downloads you can use globus and api.
Cassiicola known elsewhere causing leaf spot of cotton. The symptom is characterized by a papery center surrounded by a yellow halo. Chromone derivatives from a spongederived strain of the. Corynespora cassiicola berk and curt wei, incitant of leaf spot of brinjal, survives at the room temperatures 2830 c. Phaeohyphomycosis caused by a plant pathogen, corynespora.
Pdf the fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in malaysia hsueh, 2011. Corynespora cassiicola was pathogenic on the roots and hypocotyls of soybean seedlings grown in infested soil at ottawa. Pdf chlamydospore formation by corynespora cassiicola. A necrotic subcutaneous lesions over the dorsal aspects of both hands with purulent discharge in a patient with chronic subcutaneous infection caused by corynespora cassiicola. An updated phylogenetic classification of corynespora. Pathogenicity of corynespora cassiicola on different hosts. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china. A case of phaeohyphomycosis caused by corynespora cassiicola.
A new foliar disease, corynespora leaf spot, has been. Infection process of corynespora cassiicola tagged with gfp on hevea brasiliensis article pdf available in australasian plant pathology 435. We do not use these to store personal information about you. Genome sequences and snp analyses of corynespora cassiicola. Unlimited viewing of the articlechapter pdf and any associated supplements and. Sensitivity of corynespora cassiicola from soybean to. Suppression by red light irradiation of corynespora leaf spot. The present study combines the examination of toxins produced by c. Brazil is the worlds second major producer of coffea canephora solitary or coalesce and form extensive irregular lesions.
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